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What is Needed?

 

 

Solutions:

 

1.     Phytohemagglutin (PHA) enriched RPMI-1640 media

2.     Colcemid solution, 10ug per ml, in Hanks balance salt solution (HBSS)

3.     Hypertonic KCl, .075M

4.     Fixative, 3 parts anhydrous methanol to 1 part glacial acetic acid

5.     Giemsa working solution 1% at pH 6.8

6.     Giemsa stock solution

7.     Phosphate buffer 0.025M (3.4 g KH2PO4 per liter adjusted to pH 6.8 with KOH)

8.          Trypsin (1:250), 1% solution in water

9.         Methanol

10.     Acridine orange